- Assistant Professor of Biology, University of North Dakota , Grand Forks , ND
- Mentored Investigator, Schepens Eye Research Institute; Instructor, Harvard Medical School
- Postdoctoral Research Fellow, Schepens Eye Research Institute and Department of Ophthalmology, Harvard Medical School , Boston , MA
- Postdoctoral Research Fellow, Department of Surgery, Children's Hospital and Department of Pathology, Harvard Medical School, Boston, MA
- Graduate student, Cell & Developmental Biology Department , Oregon Health Sciences University, Portland , OR
- Research technician/Laboratory Manager, The Burnham Institute, La Jolla, CA
 Research Interests:
My current research addresses the influence of heterotypic cell-cell interactions during central nervous system development. My work is designed to test the hypothesis that neurovascular interactions stabilize the vasculature during development and under pathologic conditions. I am also investigating the role of a traditionally vascular factor, vascular endothelial growth factor (VEGF) on the regulation of neurogenesis. I am currently conducting these studies in vitro multi-cell type coculture approaches and in vivo system using transgenic mice.
Teaching Interests: Developmental Biology (BIOL 378/499) is an upper division undergraduate course (open to graduate students) that is designed to provide an overview of general developmental biology principles. The course includes the general stages of development associated with a variety of species, some of the experimental approaches used to study developmental processes, and the genetic and environmental influences that govern development.
Introduction to Biology (BIOL 150) is a team-taught lower division undergraduate course designed primarily for students majoring in Biology and other science-related disciplines. The course covers fundamental aspects of Biology at the molecular, cellular and physiological level.
Topics in Cell and Molecular Biology (BIOL 491/503) is a team-coordinated upper division undergraduate course (open to graduate students) that is designed to improve student comprehension of current topics through critical reading of published research. Faculty and students present and discuss the literature in an informal journal club format.
Selected Publications: Levenberg, S, Rouwkema, J, MacDonald, M, Garfein, ES, Kohane, D, Darland, DC, Marini, R, vanBlitterswijk, CA, Muligan, RC, D'Amore, PA, Langer, R. Engineering vascularized skeletal muscle tissue. Nature Biotechnology , 23(7):879-84, 2005 .
Arboleda-Velasquez, JF, Rampal, R, Fung, E, Darland, DC, Liu, M, Martinez, MC, Donahue, CP, Navarro-Gonzalez, MF, Libby, P, D'Amore, PA, Aikawa, M, Haltiwanger, RS, Kosik, KS. Cadasil mutations impair Notch3 glycosylation by fringe. Human Molecular Genetics , 14(12): 1631-1639, 2005.
Garcia, CM, Darland , DC , Massingham, LJ, D'Amore, PA. Endothelial cell-astrocyte interactions and TGFB are required for induction of blood-neural barrier properties, Developmental Brain Research , 152:25-38, 2004.
Morris PN, Dunmore BJ, Tadros A, Marchuk DA, Darland DC, D'Amore PA & Brindle NPJ. Functional analysis of a mutant form of the receptor tyrosine kinase Tie2 causing venous malformations. Journal of Molecular Medicine , 83:58-63, 2004.
Ding, R, Darland , DC , Parmacek , MS , and D'Amore, PA. Endothelial-mesenchymal interactions in vitro reveal molecular mechanisms of smooth muscle/pericyte differentiation. Stem Cells and Development , 13:509-520, 2004.
Darland , DC , Massingham, LJ, Smith, SR, Piek, E, Saint-Geniez, M. and D'Amore, PA. Pericyte production of cell-associated VEGF is differentiation-dependent and is associated with endothelial survival. Developmental Biology , 264:275-288, 2003.
Le Gat, L, Gogat, K, Saint-Geniez, M, Darland, D, Van Den Berghe, L, Bouquet, C, Marchant, D, Provost, A, Perricaudet, M, Menasche, M, and Abitbol, M. In vivo adenovirus-mediated delivery of a uPA/uPAR antagonist reduces retinal neovascularization in a mouse model of retinopathy. Gene Therapy , 10:2098-2103, 2003.
Hartnett , ME , Lappas, A, Darland , DC , McColm, JR, Lovejoy, S, D'Amore, PA. Retinal pigment epithelium and endothelial cell interaction causes retinal pigment epithelial barrier dysfunction via a soluble VEGF-dependent mechanism. Experimental Eye Research , 77:593-599, 2003.
Darland , DC and D'Amore, PA. TGF b is required for the formation of capillary-like structures in three-dimensional cocultures of 10T1/2 and endothelial cells. Angiogenesis , 4:11-20, 2001. |
