Developmental Neurobiology
Assistant Research Professor of Biology
Education:
B.S. 1986, Biology - Revelle College, UCSD
Ph.D. 1998 Cell and Developmental Biology - Oregon Health Sciences University
Experience:
Assistant Research Professor of Biology, University of North Dakota
Postdoctoral Research Associate, Harvard University
Research Focus:
Neurodevelopment in Zebrafish
Neural Stem Cell Regulation and Addiction Related Behavior
I am interested in finding new genes, or new functions of known genes, involved in diverse biological processes ranging from neurodevelopment and neural stem cell regulation to addiction related behavior. I use zebrafish because the genetic analysis possible is amenable to find new candidate genes and is not currently possible in other vertebrates. I am currently identifying the genes underlying mutations that affect neural stem cell regulation in the retina. Fish have the ability to generate new nervous tissue in adulthood, a process that may be applicable to treating neural injury and pathology in humans. Also, I am identifying other mutant gene products that underlie abnormal behavioral responsiveness to addictive drugs.
I house a number of wild type strain of zebrafish and hope to make the organism available to other investigators on the UND campus interested in trying new experiments with this very useful organism. In addition, I am involved in summer educational outreach programs for undergraduates and hope to provide teaching laboratory workshops on using zebrafish for genetic analysis.
Selected Publications :
Gross JM, Perkins BD, Amsterdam A, Egana A, Darland T , Matsui JI, Sciasscia S, Hopkins N and Dowling JE. Identification of Zebrafish Insertional Mutants with Defects in Visual System Development and Function. Genetics 2005, (Epub ahead of print).
Darland T and Dowling JE. Behavioral screening for cocaine sensitivity in mutagenized zebrafish. PNAS USA 2001; 98(20): 11691-6.
Link BA and Darland T . Genetic analysis of initial and ongoing retinogenesis in the zebrafish: comparing the central neuroepithelium and marginal zone. Prog Brain Res . 2001; 131: 565-77.
Bunzow JR, Sonders MS, Arttamangkul S, Harrison LM, Zhang G, Quigley DI, Darland T , Suchland KL, Pasumamula S, Kennedy JL, Olsen SB, Magenis RE, Amara SG and Grandy DK. Amphetamine, 3,4-methylenedioxymethamphetamine, lysergic acid diethylamide, and metabolites of the catecholamine neurotransmitters are agonists of a rat trace amine receptor . Mol. Pharm. 2001; 60 (6) 1181-8.
Allen CN, Jiang ZG, Teshima K, Darland T , Ikeda M, Nelson CS, Quigley DI, Yoshioka T, Allen RG, Rea MA and Grandy DK. Orphanin-FQ/nociceptin (OFQ/N) modulates the activity of suprachiasmatic nucleus neurons. J. Neurosci. (1999); 19(6): 2152-60.
Darland T , Grandy DK. The orphanin FQ system: an emerging target for the management of pain? Br. J. Anaesth . 1998; 81(1): 29-37.
Darland T , Heinricher MM, Grandy DK. Orphanin FQ/Nociceptin: a role in pain and analgesia, but so much more. Trends in Neurosci . 1998; 21(5): 215-21.
Quigley DI, McDougall J, Darland T , Zhang G, Ronnekliev O, Grandy DK, Allen RG. Orphanin FQ is the major OFQ1-17-containing peptide produced in the rodent and monkey hypothalamus. Peptides 1998;19(1):133-9.
Darland T , Leblanc GG. Immortalized Hensen's node cells secrete a factor that regulates avian neural crest cell fates in vitro . Dev Biol . 1996; 176(1): 62-75.
Leblanc GG, Holbert TE, Darland T . Role of the transforming growth factor-beta family in the expression of cranial neural crest-specific phenotypes. J Neurobiol . 1995; 26(4): 497-510.
Huang RP, Darland T , Okamura D, Mercola D, Adamson ED. Suppression of v-sis-dependent transformation by the transcription factor, Egr-1. Oncogene 1994; 9(5):1367-77.
Joh T, Darland T , Samuels M, Wu JX, Adamson ED. Regulation of epidermal growth factor receptor gene expression in murine embryonal carcinoma cells . Cell Growth Differ. 1992;3(5):315-25.
Edwards SA, Darland T , Sosnowski R, Samuels M, Adamson ED. The transcription factor, Egr-1, is rapidly modulated in response to retinoic acid in P19 embryonal carcinoma cells. Dev. Biol . 1991; 148(1): 165-73.
Darland T , Samuels M, Edwards SA, Sukhatme VP, Adamson ED. Regulation of Egr-1 and c-fos expression in differentiating embryonal carcinoma cells. Oncogene 1991; 6(8):1367-76.
Calogero A, Samuels M, Darland T , Edwards SA, Kemler R, Adamson ED. Overexpression of uvomorulin in a compaction-negative F9 mutant cell line. Dev Biol. 1991;146(2):499-508.
|
