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Dr. Alena Kubatova

Assistant Professor

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Our research involves application and development of analytical separation methods to improve understanding the origins of air pollution and its relation to climate changes. This involves the use of chromatographic techniques with various types of detectors, mainly mass spectrometric detection (MS), e.g., the instrumentation which is frequently used in research and industry. In our approach, We emphasize the importance of sample preparation method development which is essential for the correct determination of analytes in various matrices. Sample preparation is assumed to be a trivial step in the analysis, but yet it is often neglected. Our laboratory is equipped with modern extraction instrumentation employing supercritical carbon dioxide, hot pressurized water, and/or organic solvents.

Understanding atmospheric chemistry processes

Air particulate matter is known for its adverse impact on heath, as well as on ever discussed climate changes. The building blocks of particulate matter are metals, salts, organic species, and elemental carbon. While inorganics are well characterized, only limited information is available on organics.

The organics can be differentiated on polar and nonpolar species. Nonpolar and slightly polar compounds are directly related to primary emissions, and are quite well characterized using organic solvent extraction and GC/MS. However, the interest of our laboratory lies in polar organics, which are formed from the atmospheric oxidation of volatile organics species (secondary aerosol formation). Because of their reactivity, polar species are suspected to have impact both on our health and climate changes.

   

Isolation of biologically active molecules from plant matrices

Various bioactive molecules are today isolated and/or synthesized to be used in products for food and drug industry. Our work involves development of selective isolation methods, preferentially using nontoxic solvents such as carbon dioxide and water, for antioxidants, vitamins, essential oils and other important plant components. Identification of those species is performed using chromatographic techniques with mass spectrometric detection.

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REPRESENTATIVE PUBLICATIONS

Kubátová A., Dronen L.C., Picklo M.J. , Hawthorne S. B Mid-polarity and nonpolar wood smoke PM fractions deplete glutathione in RAW 264.7 macrophages, Chem. Res. Toxicol. July 2005 in press.

Ibañez E., Kubátová A., Señoráns F. J., Cavero S., Reglero G., Hawthorne, S.B. Subcritical water extraction of antioxidant compounds from rosemary plants. J. Agric. Food Chem.2003, 51, 375–382.

Kubátová A., Vermeylen R., Clayes M., Cafmeyer J. Maenhaut, W. Roberts G., Artaxo P. Carbonaceous aerosol characterization in the Amazon basin, Brazil: Novel dicarboxylic acids and related compounds. Atmos. Environ. 2000, 34, 5037–5051.

Kubátová A., Lagadec A.J.M., Miller, D.J., Hawthorne S.B. Selective extraction of oxygenates from savory and peppermint using subcritical water. Flavour Fragr. J. 2000, 16, 64–73.

 

 

 

 

 

Chemistry Department
Abbott Hall Room 236
151 Cornell Street Stop 9024
Phone: 701-777-2741
Fax: 701-777-2331
Email: info@chem.und.edu